| Experimental Details - HuPA_00007 |
| Experiment type |
Mass spectrometry |
| Short description |
Platelet subproteome analysis |
| Experimental description |
Commercially available kits were used for crude fractionation of cells into different compartments, such as membranes, cytosol, nucleus and cytoskeleton. Platelet proteome, activated and resting states, was characterized and compared. |
| Principal Investigator's Name | Dr. Gerard Cagney |
| Title | Assistant Professor |
| E-mail | gerard.cagney@ucd.ie |
| Address | Conway Institute
University College Dublin
Belfield, Dublin 4 |
| Country | Ireland |
| Lab URL | http://proteomics.ucd.ie/index.html |
| Data submitted by | Mathew Sullivan |
| Title | BioInformatics Group Leader |
| E-mail | matthew.sullivan@ucd.ie |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
|
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
No |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
1.5 Da |
| Mass tolerance used for database searching (MS/MS) |
0.5 Da |
| Database used for searching |
RefSeq |
| Search engine used |
SEQUEST |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
