| Experimental Details - HuPA_00001 |
| Experiment type |
Mass spectrometry |
| Short description |
Brain proteome analysis |
| Experimental description |
Brain tissue was homgenised and separated out using 2D gel electrophoresis. Protein spots differing between schizophrenia bipolar disorder and major depression, in comparison with controls, were identified using MALDI-TOF or LC-MS/MS |
| Principal Investigator's Name | Dr. Michael Dunn |
| Title | Professor |
| E-mail | michael.dunn@ucd.ie |
| Address | UCD Conway Institute
University College Dubl
Belfield, Dublin 4 |
| Country | Ireland |
| Lab URL | http://www.ucd.ie/conway |
| Data submitted by | Dr. Kyla Pennington |
| Title | Postdoctoral Researcher |
| E-mail | kyla.pennington@ucd.ie |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16637010
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
1. ESI [PSI:1000073]
2. MALDI [PSI:1000075] |
| Fragmentation method |
1. CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
100 ppm |
| Mass tolerance used for database searching (MS/MS) |
100 ppm |
| Database used for searching |
ProteinLynx |
| Search engine used |
Mascot |
| Download MS raw dataset |
|
| Download processed files |
|
