| Experimental Details - HuPA_00003 |
| Experiment type |
Mass spectrometry |
| Short description |
B Cell proteome analysis |
| Experimental description |
Protein indentifications are made by replicate 2D-LC-MS/MS analysis of tryptic peptides from detergent solubilized B cell membrane proteins, as well as replicate LC-MS/MS analysis of single off-line strong cation exchange chromatography (SCX) fractions |
| Principal Investigator's Name | Dr. David Muddiman |
| Title | Professor |
| E-mail | david_muddiman@ncsu.edu |
| Address | North Carolina State University
608E Cox Hall
Raleigh, NC 27695 |
| Country | USA |
| Lab URL | http://www.ncsu.edu/chemistry/dcm/index.html |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
15797518
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
|
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
No |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
0.5 Da |
| Mass tolerance used for database searching (MS/MS) |
0.5 Da |
| Database used for searching |
RefSeq |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
