| Experimental Details - HuPA_00005 |
| Experiment type |
Mass spectrometry |
| Short description |
Plasma proteome analysis |
| Experimental description |
Plasma was depleted first with the top 12 abundance proteins and then the rest of proteins were separated with multi-lectin affinity column to have two pools (bound and unbound). Each pool was digested with trypsin with reduction and alkylation. The digested peptides were analyzed by LC-MS. This dataset represents unbound pool. |
| Principal Investigator's Name | Dr. William Hancock |
| Title | Professor |
| E-mail | wi.hancock@neu.edu |
| Address | Bradstreet Chair Barnett Institute
Department of Chemistry and Chemical Biology, Northeastern University 360 Huntington Avenue
Boston, MA 2115 |
| Country | USA |
| Data submitted by | Dr. Shiaw-Lin (Billy) Wu |
| Title | Research Associate Professor |
| E-mail | si.wu@neu.edu |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
1000 ppm |
| Mass tolerance used for database searching (MS/MS) |
1000 ppm |
| Database used for searching |
NCBI nr |
| Search engine used |
SEQUEST |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
