| Experimental Details - HuPA_00008 |
| Experiment type |
Mass spectrometry |
| Short description |
Serum proteome analysis |
| Experimental description |
Immunoglobulins were removed from serum using protein A/G, and the remaining proteins were digested with trypsin. Resulting peptides were separated by strong cation exchange chromatography into distinct fractions prior to analysis. |
| Principal Investigator's Name | Dr. Richard Smith |
| Title | Laboratory Fellow and Resource Director |
| E-mail | dick.smith@pnl.gov |
| Address | Proteomics Research Resource for Integrative Biology
P.O. Box 999, K8-91
Richland, WA 99352 |
| Country | USA |
| Lab URL | http://ncrr.pnl.gov |
| Data submitted by | Samuel O Purvine |
| Title | Senior Research Scientist |
| E-mail | samuel.purvine@pnl.gov |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
12543931
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
|
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
No |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
3.0 Da |
| Mass tolerance used for database searching (MS/MS) |
0.0 Da |
| Database used for searching |
RefSeq |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
