| Experimental Details - HuPA_00009 |
| Experiment type |
Mass spectrometry |
| Short description |
Serum proteome analysis |
| Experimental description |
Albumin/IgG depleted serum was fractionated by Protein-based 2D-LC. Collected fractions were analysed by LC-MS/MS |
| Principal Investigator's Name | Dr. Jenny Van Eyk |
| Title | Director |
| E-mail | jvaneyk1@jhmi.edu |
| Address | Johns Hopkins Bayview NHLBI Proteomics Center 5200 Eastern Ave., MFL Center Tower 601 Baltimore, MD 21224 |
| Country | USA |
| Data submitted by | Shijun Sheng |
| Title | Proteomics Analyst |
| E-mail | ssheng1@jhmi.edu |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16188874
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
|
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
No |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
1.5 Da |
| Mass tolerance used for database searching (MS/MS) |
0.8 Da |
| Database used for searching |
RefSeq |
| Search engine used |
Mascot |
| Download MS raw dataset |
|
| Download processed files |
|