| Experimental Details - HuPA_00012 |
| Experiment type |
Mass spectrometry |
| Short description |
Her2/neu tyrosine phosphoproteome anlaysis |
| Experimental description |
Three state SILAC experiment where tyrosine phosphorylation events in Her2/neu and downstream proteins were measured by anti-phosphotyrosine antibody pulldown followed by LC-MS/MS |
| Principal Investigator's Name | Dr. Philip Cole |
| Title | Professor |
| E-mail | pcole@jhmi.edu |
| Address | Johns Hopkins University
Hunterian 316, 725 N. Wolfe St.
Baltimore, MD 21205 |
| Country | USA |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16785428
|
| Sample source |
| Tissue: |
|
| Cell line: |
NIH3T3
|
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
13C6-arginine and 13C6,15N4-arginine (please note that this peptide does not contain Arg and therefore was not labelled with these isotopes) |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
0.3 Da |
| Mass tolerance used for database searching (MS/MS) |
0.3 Da |
| Database used for searching |
RefSeq |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
