| Experimental Details - HuPA_00013 |
| Experiment type |
Fusion proteins for subcellular localization |
| Short description |
Organellar proteomic analysis |
| Experimental description |
HeLa cells were transfected with gi:23271560 (nucleotide gi:23271559) in pEGFP-N1 vector (Clontech). 48h post-transfection cells were fixed with 3%PFA in PBS for 20min RT. Cells were stained for GM130 (Transduction laboratories) followed by Cy3-coupled secondary antibody (Jackson Immunoresearch, West Grove, PA, USA). |
| Principal Investigator's Name | Dr. Ragna Rönnholm |
| Title | University lecturer |
| E-mail | ronnholm@cc.helsinki.fi |
| Address |
Department of Biological and Environmental Sciences
University of Helsin, FI- 14 |
| Country | Finland |
| Data submitted by | Maarit Takatalo |
| Title | Student |
| E-mail | maarit.takatalo@helsinki.fi |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16691549
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
