| Experimental Details - HuPA_00015 |
| Experiment type |
Mass spectrometry |
| Short description |
HeLa phosphoproteome anlaysis |
| Experimental description |
HeLa cells were stimulated with epidermal growth factor (EGF) and temporal profiles were observed. |
| Principal Investigator's Name | Dr. Matthias Mann |
| Title | Professor |
| E-mail | mmann@biochem.mpg.de |
| Address | Max Planck Institute for Biochemistry
Department of Proteomics and Signal Transduction
Martinsried, D- 82152 |
| Country | Germany |
| Lab URL | http://www.biochem.mpg.de/en/research/rd/mann/people/index.html |
| Data submitted by | Florian Gnad |
| Title | Ph.D. Student |
| E-mail | gnad@biochem.mpg.de |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
17081983
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
SILAC labeling method (L-lysine-2H4, L-lysine-U-13C6-15N2, L-arginine-U-13C6, L-arginine-U-13C6-15N4) |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
DTT and iodoacetamide |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
|
| Mass tolerance used for database searching (MS/MS) |
|
| Database used for searching |
IPI |
| Search engine used |
Mascot |
| Download MS raw dataset |
|
| Download processed files |
|
