| Experimental Details - HuPA_00018 |
| Experiment type |
Mass spectrometry |
| Short description |
Hair shaft proteome analysis |
| Experimental description |
Insoluble proteins from human hair shaft was extracted with extensive solubilization and reduced, alkylated, and digested. Digested peptide mixture was fractionated with off-link SCX before nanoLC-MS/MS. |
| Principal Investigator's Name | Dr. Robert H. Rice |
| Title | Professor |
| E-mail | rhrice@ucdavis.edu |
| Address | University of California, Davis
One Shields Avenue
Davis, CA 95616 |
| Country | USA |
| Lab URL | http://www.envtox.ucdavis.edu/Faculty_Personal_Web_Pages/rhrice_files/NewRice.htm |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16446289
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
Reduction w/ 20mM DTE in 2%SDS, 50mM phsophate buffer and alkylation w/ 40mM IoAA |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
2.5 Da |
| Mass tolerance used for database searching (MS/MS) |
1 Da |
| Database used for searching |
IPI |
| Search engine used |
SEQUEST |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
