| Experimental Details - HuPA_00019 |
| Experiment type |
Mass spectrometry |
| Short description |
Sulfation analysis |
| Experimental description |
Mass spectrometry-based approach in combination with direct DNA sequence analysis was used to fully identify TTR variants. To identify TTR variants and posttranslational modifications, Immunoprecipitation to isolate TTR from serum, and electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry (MS) peptide mapping were done. |
| Principal Investigator's Name | Dr. Mark McComb |
| Title | Professor |
| E-mail | mccomb@bu.edu |
| Address | Boston University School of Medicine
670 Albany Street, suit 504
Boston, MA 2118 |
| Country | USA |
| Lab URL | http://www.bumc.bu.edu/cardiovascularproteomics |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
12876326
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
|
| Reduction and Alkylation |
|
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
|
| Mass tolerance used for database searching (MS/MS) |
|
| Database used for searching |
SwissProt |
| Search engine used |
PROID |
| Download MS raw dataset |
|
| Download processed files |
|
