| Experimental Details - HuPA_00021 |
| Experiment type |
Mass spectrometry |
| Short description |
Urine phosphoproteome analysis |
| Experimental description |
50 mL volumes of urine was collected from 32 years healthy male (one voiding during the morning). Urine was centrifuged in a fixed-angle centrifuge for 30 minutes at 4500 x g. The supernatant were desalted with membrane filter(MWCO 5kDa) followed by reduction and alkylation followed by Nano-LC-ESI-MS/MS analysis. |
| Principal Investigator's Name | Dr. Young-Ki Paik |
| Title | Associate Professor |
| E-mail | paikyk@yonsei.ac.kr |
| Address | Yonsei University
Yonsei University
Sudaemoon-ku, Seoul 120 |
| Country | South Korea |
| Data submitted by | Min-Seok Kwon |
| Title | Mr. |
| E-mail | kwonms@proteomix.org |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
1.5 Da |
| Mass tolerance used for database searching (MS/MS) |
1 Da |
| Database used for searching |
IPI |
| Search engine used |
SEQUEST |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
