| Experimental Details - HuPA_00022 |
| Experiment type |
Mass spectrometry |
| Short description |
Lung proteome analysis |
| Experimental description |
Bronchoalveolar lavage drawn from five healthy patients, supernatant and cellular fractions separated, supernatant fraction digested in solution with trypsin, analyzed by nanoUPLC-MS/MS in data dependent fashion |
| Principal Investigator's Name | Dr. Thomas Neubert |
| Title | Associate Professor |
| E-mail | neubert@saturn.med.nyu.edu |
| Address | Skirball Institute for Biomolecular Medicine
New York University Medical Center, 540 First Ave., Lab 5-18
New York, NY 10016 |
| Country | USA |
| Data submitted by | Dr. Helene Cardasis |
| Title | Postdoctoral Fellow |
| E-mail | cardasis@saturn.med.nyu.edu |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
0.3 Da |
| Mass tolerance used for database searching (MS/MS) |
0.7 Da |
| Database used for searching |
NCBI |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
