| Experimental Details - HuPA_00026 |
| Experiment type |
Mass spectrometry |
| Short description |
B Cell proteome analysis |
| Experimental description |
Proteins affected by anti-mIgM stimulation were identified using 2-DE-based proteomics. We investigated the proteome profiles of stimulated and nonstimulated Ramos B-cells at eight time points during 5 d. |
| Principal Investigator's Name | Dr. Mauno Vihinen |
| Title | Professor of Bioinformatics |
| E-mail | mauno.vihinen@uta.fi |
| Address | Institute of Medical Technology
FI-33014 University of Tampere
Biokatu, Tampere |
| Country | Finland |
| Lab URL | http://bioinf.uta.fi/BcellProteome/ |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16947121
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
SYPRO Ruby gel stain (Molecular Probes) |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
DTT and IAA |
| Mass spectrometer |
|
| Ionization method |
MALDI [PSI:1000075] |
| Fragmentation method |
PSD [PSI:1000135] |
| Mass tolerance used for database searching (MS) |
50-150 ppm |
| Mass tolerance used for database searching (MS/MS) |
80-150 ppm |
| Database used for searching |
MSDB |
| Search engine used |
Mascot |
| Download MS raw dataset |
|
| Download processed files |
|
