| Experimental Details - HuPA_00028 |
| Experiment type |
Mass spectrometry |
| Short description |
Histone modification analysis |
| Experimental description |
293 HEK cells were collected and lysed and the soluble proteins cleared by ultracentrifugation. Histones were purifed from solution using rabbit polyclonal antibodies to histone H2A1.1 coupled to magnetic beads. The purified histones were isolated using 1-D SDS-PAGE and the histone bands excised. The histones were digested in an in-gel digestion and the peptides extracted from the gel slices. The isolated peptides were then run through a reverse-phase HPLC column into an ESI front-end to a QStar-XL. |
| Principal Investigator's Name | Dr. Alma Burlingame |
| Title | Professor |
| E-mail | alb@cgl.ucsf.edu |
| Address | Mass Spectrometry Facility
521 Parnassus Ave., C-18, Mass Spectrometry Facility, University of California
San Francisco, CA 94143-0446 |
| Country | USA |
| Lab URL | http://msf.ucsf.edu/ |
| Data submitted by | Dr. Feixia Chu |
| Title | Postdoctoral Fellow |
| E-mail | fchu@cgl.ucsf.edu |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
| Tissue: |
|
| Cell line: |
293 HEK
|
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
No |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
200 ppm |
| Mass tolerance used for database searching (MS/MS) |
0.2 Da |
| Database used for searching |
SwissProt |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
