| Experimental Details - HuPA_00030 |
| Experiment type |
Mass spectrometry |
| Short description |
Histone modifications analysis |
| Experimental description |
Human small cell lung cancer cells exposed to the HDACi PXD101. Triplicate analysis by LC-MS/MS. Data analysis by VEMS software. |
| Principal Investigator's Name | Dr. Ole Nørregaard Jensen |
| Title | Professor |
| E-mail | jenseno@bmb.sdu.dk |
| Address | University of Southern Denmark
Institute of Biochemistry and Molecular Biology, Campusvej 55,
Odense M, DK- 5230 |
| Country | Denmark |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16627869
|
| Sample source |
| Tissue: |
|
| Cell line: |
SCLC OC-NYH
|
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
|
| Reduction and Alkylation |
yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
0.6 Da |
| Mass tolerance used for database searching (MS/MS) |
0.6 Da |
| Database used for searching |
Human histone database |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
