| Experimental Details - HuPA_00031 |
| Experiment type |
Mass spectrometry |
| Short description |
Phosphoproteome analysis |
| Experimental description |
100ug of total cell lysate was run on the SDS-PAGE and the whole lane was in-gel digested with trypsin. From the exracted peptide mixture, phosphopeptides were enriched with TiO2. |
| Principal Investigator's Name | Dr. Blagoy Blagoev |
| Title | Assistant Professor |
| E-mail | bab@bmb.sdu.dk |
| Address | University of Southern Denmark
Institute of Biochemistry and Molecular Biology, Campusvej 55,
Odense M, DK- 5230 |
| Country | Denmark |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
10 ppm |
| Mass tolerance used for database searching (MS/MS) |
0.6 Da |
| Database used for searching |
IPI |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
