| Experimental Details - HuPA_00036 |
| Experiment type |
Mass spectrometry |
| Short description |
Ovary proteome analysis |
| Experimental description |
Proteins were extracted from microdissected tissue from normal ovary using 8 M urea, reduced, alkylated, digested and analyzed CIEF-LC-MS/MS |
| Principal Investigator's Name | Dr. Brian Balgley |
| Title | Director of Proteomics |
| E-mail | brian.balgley@calibrant.com |
| Address | Calibrant Biosystems
910 Clopper Rd. Ste 220N
Gaithersburg, MD 20878 |
| Country | USA |
| Data submitted by | Dr. Wejie Wang |
| Title | Scientist |
| E-mail | weijie.wang@calibrant.com |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
1.5 Da |
| Mass tolerance used for database searching (MS/MS) |
0.4 Da |
| Database used for searching |
SwissProt |
| Search engine used |
OMSSA |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
