| Experimental Details - HuPA_00037 |
| Experiment type |
Mass spectrometry |
| Short description |
Adipose derived stem cell analysis |
| Experimental description |
Primary human adipose derived stem cells were cultured in the undifferentiated and adipocyte differentiated states. Secreted proteins from each were obtained in serum free conditioned medium, separated by 2D PAGE, and analyzed by MS. |
| Principal Investigator's Name | Dr. Jeffrey Gimble |
| Title | Professor |
| E-mail | Gimblejm@pbrc.edu |
| Address | Pennington Biomedical Research Center
6400 Perkins Road
Baton Rouge, LA 70808 |
| Country | USA |
| Data submitted by | Dr. Indu Kheterpal |
| Title | Assistant Professor |
| E-mail | indu.kheterpal@pbrc.edu |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
17018519
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
0.2 Da |
| Mass tolerance used for database searching (MS/MS) |
0.2 Da |
| Database used for searching |
RefSeq |
| Search engine used |
Mascot |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
