| Experimental Details - HuPA_00038 |
| Experiment type |
Mass spectrometry |
| Short description |
Bone marrow stromal cell proteome analysis |
| Experimental description |
Bone marrow stromal cells from normal individuals and leukemia patients were culture-expanded; total proteins were extracted; they were reduced, and alkylated with acrylamide; digested with trypsin; and labeled with iTRAQ reagents, followed by protein identification and relative quantification using 2-D LC MS/MS. |
| Principal Investigator's Name | Dr. Beerelli Seshi |
| Title | Head, Hematopathology |
| E-mail | bseshi@labiomed.org |
| Address | Harbor-UCLA Medical Center and
Los Angeles Biomedical Research Institute
Torrance, CA 90502 |
| Country | USA |
| Lab URL | http://www.labiomed.org/stromalcell/index.htm |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16947122
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
iTRAQ used for labeling |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
0.15 Da |
| Mass tolerance used for database searching (MS/MS) |
0.1 Da |
| Database used for searching |
CDS, SwissProt and IPI Human |
| Search engine used |
ProQuant software v1.1 |
| Download MS raw dataset |
|
| Download processed files |
|
