| Experimental Details - HuPA_00041 |
| Experiment type |
Mass spectrometry |
| Short description |
Saliva proteome analysis |
| Experimental description |
Whole and parotid salivas were donated by adult healthy subjects. Saliva proteins were concentratred by TCA/acetone and separated on 2D electrophoretic gels. Excised spots were identified after tryptic digestion. |
| Principal Investigator's Name | Dr. Eric Dransfield |
| Title | Principal Investigator |
| E-mail | eric.dransfield@wur.nl |
| Address | INRA
WCFS, Diedenweg 20
Wageningen, AN 6700 |
| Country | The Netherlands |
| Data submitted by | Martine Morzel |
| Title | Research scientist |
| E-mail | Martine.Morzel@dijon.inra.fr |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
16944961
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
None |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
Yes |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
1.4 Da |
| Mass tolerance used for database searching (MS/MS) |
0.8 Da |
| Database used for searching |
Swiss-Prot/Trembl |
| Search engine used |
Bioworks 3.1 |
| Download MS raw dataset |
Download |
| Download processed files |
Download |
